Description
• These cell lines stably express one or more reporter molecules.
NIH 3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibitedand are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now losttheir contact inhibition. The established NIH/3T3 line was subjected to more than 5 serial cycles ofsubcloning in order to develop a subclone with morphologic characteristics best suited for transformationassays. The "3T3" designation refers to the abbreviation of "3-day transfer, inoculum 3 x 105 cells." TheNIH3T3 cell line is one of the most commonly used fibroblast cell lines. Our NIH3T3/GFP cell linestably expresses GFP and blasticidin-resistant genes. Both GFP and blasticidin-resistant genes areintroduced into parental NIH3T3 cells using lentivirus